Article Type
Article
Abstract
Seventy-three Staphylococcus spp. isolates were recovered from bacterial samples including poultry, foods, sewage, and soil. Antibiotic susceptibility tests were conducted on all environmental isolates for Methicillin and Vancomycin. The results showed that 39 Staphylococcus spp. isolates (53.42%) were resistant to Methicillin, while 26 isolates (35.61%) were resistant to Vancomycin. The minimum inhibitory concentration (MIC) range of Vancomycin was found to be between 2-512 µg/mL. Plasmid DNA extraction from Methicillin and Vancomycin-resistant isolates revealed that some isolates had one plasmid band. Detection of Vancomycin resistance genes (vanA and vanB) showed the presence of the vanB gene in isolate No. ESf44 with a MIC of ≥ 64 µg/mL, isolated from raw milk. The study did not detect the presence of the vanA gene in other Vancomycin-resistant isolates studied. This investigation aimed to explore the vanB gene within the isolated MRSA strains from food, sewage water, and soil in Baghdad.
Keywords
Staphylococcus spp., Vancomycin resistance genes and Bacterial isolates.
Recommended Citation
Hadi, Zina A.S and Al-Marjani, Mohamed F.
(2020)
"Detection of Van B gene in Staphylococcus spp. Isolated from Food in Baghdad/Iraq,"
Al-Esraa University College Journal for Medical Sciences: Vol. 2:
Iss.
1, Article 1.
DOI: https://doi.org/10.70080/2790-7937.1028