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Article Type

Article

Abstract

This study proposed to analyze chloramphenicol (CPA), sulfamethoxazole (SMX), and sulfanilamide (SNA) drugs by reverse-phase liquid chromatography method in the form of pharmaceutical preparations and synthetic mixture. For this method, column C18-ODS (25 cm x 4.6 mm) was used with an isocratic elution mobile phase composed of methanol and 2% orthophosphoric acid, fluorescent detector (Ex= 310 nm, Em= 440 nm). It was found that the time of retention for CPA, SMX, and SNA was 8.99 ± 0.02, 10.76 ± 0.2, and 8.21 ± 0.2 min, respectively. The suggested method has been validated for limit of detection, limit of quantitation, linearity, Accuracy, and Precision. The method is sensitive with a detection limit (0.016 ppm, 0.044 ppm, and 0.184 ppm) for CPA, SMX, and SNA, respectively. The method accuracy is 100.0%, and the precision of this method reflected by the Coefficient of variation (CV) for the repeats is (0.002, 0.002, and 0.0025) for CPA, SMX, and SNA, respectively. For routine analysis purposes, this method can monitor the drugs in the ingested pharmaceutical preparations.

Keywords

HPLC, Chloramphenicol, Sulphamethoxazole, Sulfanilamide

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