Biological Activity of Rats Treated with Riboflavin in Polymeric PLGA as Injectable Form

Authors

Sarah A. Hamood, Al-Esraa University College, Biomedical Engineering Dept., Baghdad / IraqFollow
Jabar A. Faraj, Al-Mustaqbal University College, Babylon / IraqFollow
Ziad T. Al-Dahan, Al-Nahrain University, College of Engineering, Dept. of Biomedical Engineering, Baghdad / IraqFollow

Article Type

Article

Abstract

The current study aims to optimize a fast, reproducible, reliable, and effective technique for estimating the biological activity of Vitamin B2 in injectable dose form using reversed-phase high-performance liquid chromatography (RP-HPLC). A biodegradable polymer, PLGA (Poly (lactic-co-glycolic acid) 50:50 type, approved as an active ingredient for parenteral administration, was used to load the vitamin in the injection volume of 1mg. Rats, both male and female, were employed as the animal models. Each animal was hosted for a duration of 40 days, and the activity of vitamin B2 was monitored at rapid intervals over six weeks using an HPLC device on plasma samples. The results indicated that all formulation batches maintained consistent response levels throughout the forty days, with the best formulation demonstrating optimal drug bioavailability within the permitted range. The best performance was observed with Fabs reaching 108 percent.

Keywords

Micro-particles, PLGA, HPLC, Pharmacokinetics, Vitamin B2, Riboflavin, Drug Delivery System, In vivo Release

Recommended Citation

Hamood, Sarah A.; Faraj, Jabar A.; and Al-Dahan, Ziad T. (2023) "Biological Activity of Rats Treated with Riboflavin in Polymeric PLGA as Injectable Form," Al-Esraa University College Journal for Medical Sciences: Vol. 4: Iss. 5, Article 6.
DOI: https://doi.org/10.70080/2790-7937.1020