Molecular Study of the Agr Gene in Multidrug-Resistant (MDR) and Extensively Drug-Resistant (XDR) in the S. aureus

Authors

Mays Ali Salim, Middle Technical University, College of Health & Medical Technology, Medical Lab. Tech. Department, Baghdad, IraqFollow
Estabraq Ali Maklef, Middle Technical University, College of Health & Medical Technology, Medical Lab. Tech. Department, Baghdad, Iraq
Montaha Abdul-Karim AL-Saffar, Middle Technical University, Institute of Medical Technology, Community Health Department, Baghdad, Iraq

Article Type

Original Study

Abstract

Staphylococcus aureus a gram-positive coccus and one of the most important opportunistic bacteria that can lead to nosocomial infections. The purpose of this study is to explore the distribution and prevalence of the Agr gene in multidrug-resistance (MDR) and extensively drug-resistant (XDR) S. aureus. 62 isolates performed antibiotic sensitivity test. The isolates showed 66.12% resistance to clindamycin with MIC at range≥4, erythromycin 77.41% with MIC at range≥8, ciprofloxacin 19.35% with MIC at range≥4, oxacillin 75.80% with MIC at range≥4, cefoxitin 45.16% with MIC at range≥8, gentamycin 29.03% with MIC at range≥16, nitrofurantoin 14.51% and MIC at range≥128, and tetracycline 75.80% MIC at range≥16. Following the extraction of genomic DNA from the bacterial isolates, the presence of the Agr gene was confirmed. Using specific primers, the Agr genes (Agr1, Agr2, Agr3, and Agr4) were successfully amplified and detected in S. aureus.

Keywords

Staphylococcus aureus, Agr gene, Multi-drug-resistant (MDR), Extensively drug-resistant (XDR), Antibiotic sensitivity test

Recommended Citation

Salim, Mays Ali; Maklef, Estabraq Ali; and AL-Saffar, Montaha Abdul-Karim (2024) "Molecular Study of the Agr Gene in Multidrug-Resistant (MDR) and Extensively Drug-Resistant (XDR) in the S. aureus," Al-Esraa University College Journal for Medical Sciences: Vol. 5: Iss. 8, Article 7.
DOI: https://doi.org/10.70080/2790-7937.1039